[Histonet] c-fos

[Jackie M O'Connor]

Thimerosol is a mercury based preservative.  Bad stuff. 




"Turner, Scott" <SCOTT.TURNER <@t> SPCORP.COM> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
04/26/2007 01:09 PM

To
<histonet <@t> lists.utsouthwestern.edu>
cc

Subject
RE: [Histonet] c-fos






Are you sure it's "thermosol" and not "thimerosal"?  Thimerosal is a 
commonly used preservative and can be found on the Sigma website (CAS 
Number: 54-64-8).  Thermosol is a process used in commercial dyeing.

Scott Turner
Schering-Plough Biopharma
(formerly DNAX Research Institute)
Palo Alto, CA



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Delatour 
Benoît
Sent: Thursday, April 26, 2007 10:51 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] c-fos


I also confirm that fos IHC can be performed on frozen sections after 
fixation-cryoprotection without thermosol. If you really need a 
preservative you can use sodium diazide 
(
http://www.sigmaaldrich.com/catalog/search/ProductDetail?ProdNo=71289&Brand=FLUKA
).

Benoît

histonet-request <@t> lists.utsouthwestern.edu a écrit :
> Message: 8
> Date: Thu, 26 Apr 2007 08:56:36 -0400
> From: jaina.negandhi <@t> sickkids.ca
> Subject: [Histonet] c-fos staining
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> 
> <OFD12451E8.B37BC3B3-ON852572C9.004719B4-852572C9.004719BD <@t> sickkids.ca>
> 
> Content-Type: text/plain; charset=US-ASCII
>
> I will be carrying out c-fos straining, and according to the protocol 
> I am following, I need to preserve my tissue in a mixture of succrose 
> and Thermosol. Thermosol is a preservative, however, I can't seem to 
> find it anywhere (i.e sigma, fluka etc.) Is there another 
> chemical/preservative that I can use instead, or even another 
> technique?
>
> Jaina
> 

-- 
Laboratoire de Neurobiologie de l'Apprentissage,
de la Mémoire & de la Communication,
NAMC, CNRS UMR 8620, Bât 446
Université Paris-Sud
91405 Orsay Cedex, FRANCE
Email  benoit.delatour <@t> u-psud.fr
Web    http://www.namc.u-psud.fr


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