[Histonet] Re: Use of 1% AgNO3 in gross room

rsrichmond <@t> aol.com rsrichmond <@t> aol.com
Thu Apr 26 13:17:47 CDT 2007

Bernice Frederick at theRobert H. Lurie Cancer Center, Northwestern 
University, Chicago suggests:

>>After the India ink, a quick dip in Bouin's helps to fix the ink 
really well!<<

Widely done, though I've never found it helped much - it's much more 
important to blot the specimen thoroughly dry before inking it.

Bouin's fixative is messy and has environmental hazards. Acetone - 
major fire hazard - is sometimes used after inking also. Supposedly 2% 
acetic acid (white vinegar diluted about half and half) works quite as 
well and is not hazardous, but I've never tried it.

Bob Richmond
Samurai Pathologist
Knoxville TN
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