[Histonet] c-fos

[Delatour Benoît]

I also confirm that fos IHC can be performed on frozen sections after 
fixation-cryoprotection without thermosol. If you really need a 
preservative you can use sodium diazide 
(http://www.sigmaaldrich.com/catalog/search/ProductDetail?ProdNo=71289&Brand=FLUKA).

Benoît

histonet-request <@t> lists.utsouthwestern.edu a écrit :
> Message: 8
> Date: Thu, 26 Apr 2007 08:56:36 -0400
> From: jaina.negandhi <@t> sickkids.ca
> Subject: [Histonet] c-fos staining
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> 	<OFD12451E8.B37BC3B3-ON852572C9.004719B4-852572C9.004719BD <@t> sickkids.ca>
> 	
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>
> I will be carrying out c-fos straining, and according to the protocol I am
> following, I need to preserve my tissue in a mixture of succrose and
> Thermosol. Thermosol is a preservative, however, I can't seem to find it
> anywhere (i.e sigma, fluka etc.) Is there another chemical/preservative
> that I can use instead, or even another technique?
>
> Jaina
>   

-- 
Laboratoire de Neurobiologie de l'Apprentissage,
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