[Histonet] AFB Contamination

Liz Chlipala liz <@t> premierlab.com
Tue Apr 24 15:27:22 CDT 2007


There is a ton of mycobacterium in tap water and in the plumbing system.  We
always use filtered water in our water bath for AFB staining.  Check out
Freidas book and staining for AFB.  The culprit is probably your waterbath,
but it could also be your AFB stain.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz <@t> premierlab.com
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Tracey.Lenek <@t> CLS.ab.ca
Sent: Tuesday, April 24, 2007 2:14 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] AFB Contamination


We are experiencing transient false positive ZN staining on skin biopies.
Today a slide had clumped AFB over the majority of the slide where no tissue
was present.  Previous to this, only one or two bacilli were noted on the
Has anyone investigated and found a contaminated waterbath or perhaps the
Kinyoun's is the culprit as our  lab reuses both?  Any help would be


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