[Histonet] hot air oven for drying slides temp

Fri Apr 20 07:54:55 CDT 2007

For those interested it is Volume 28, Number 1, March 2005. 

Jeanine Bartlett
Infectious Disease Pathology Branch
(404) 639-3590 
jeanine.bartlett <@t> cdc.hhs.gov

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of patsy
ruegg
Sent: Friday, April 20, 2007 8:37 AM
To: 'Diana McCaig'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] hot air oven for drying slides temp

There was an article in JOH last year I believe on the effects of slide
drying temps on IHC, it was very interesting and yes high temps can
affect some antigens.  I keep my drying oven at 60dc or below.
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #216
Aurora, CO 80010
720-859-4060
fax 720-859-4110
pruegg <@t> ihctech.net
www.ihctech.net
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Diana
McCaig
Sent: Friday, April 20, 2007 6:02 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] hot air oven for drying slides temp

I was under the impression that the temp of the drying oven in a stainer
or a hot air oven if stained seperately should be aroung 60
degrees--just above the melting point of the paraffin.  I have recently
been in a lab where they dry their routine and special stain slides at
95 degrees.  The leave their immunos at room temp over night.  Their
seems to be no complaints by the pathologists with the temp that high
but I was wondering what the averge temp others are using.
Thanks Diana
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