[Histonet] testes fixation/ staining

[Elizabeth M Heimrich]

The only thing I can say is that Picric acid left in tissue ultimately 
affects the staining of the nuclei if not neutralized with EtOH 
saturated w/ Li2CO3.  There is a much better fixative of testes 
'Modified Davidson's Fluid'.  The pathologist I worked with, Dr Diane 
Creasy, published her findings in TOXICOLOGIC PATHOLOGY vol. 30, no 4, 
pp524-533, 2002, entitled _Fixation of Testes and Eyes Using a Modified 
Davidson's Fluid: Comparison with Bouin's Fluid and Conventional 
Davidson's Fluid._  This article describes the recipe for mDavidson's 
and compares it to other Fixatives routinely used.
Best of Luck!
Beth