[Histonet] RE: [IHCRG] PDGF alpha, beta and receptors

[Patsy Ruegg]

Mary,

I use PDGF B from Lab Vision RB-9257 at 1:100 steam AR for 25 min. with
citrate buffer, hrp rab labeled polymer for 30 min., DAB or AEC (I do this
on skin samples) for 10 min.  I have not tried the others.

Patsy

 

  _____  

From: ihcrg <@t> googlegroups.com [mailto:ihcrg <@t> googlegroups.com] On Behalf Of
Vaughan, Mary
Sent: Monday, April 16, 2007 6:58 AM
To: Histonet
Subject: [IHCRG] PDGF alpha, beta and receptors

 

good morning everyone-

 

I have a proposed project that involves staining of PDGF alpha, beta, and
the receptor for each. Does anyone have these protocols worked out? I'd
appreciate some help, thanks.

 

[PDGF-A, PDGFR-a, PDGF-B, PDGFR-b]

 

 

Best Regards,

Mary Vaughan HT (ASCP)

Research Specialist

Roswell Park Cancer Institute

ASB-212

Elm & Carlton Streets

Buffalo, N Y 14263

phone: 716.845.3006

FAX: 716.845.3489

email: mary.vaughan <@t> roswellpark.org

 


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