[Histonet] RE: [IHCRG] PDGF alpha, beta and receptors

Patsy Ruegg pruegg <@t> ihctech.net
Wed Apr 18 13:26:10 CDT 2007


I use PDGF B from Lab Vision RB-9257 at 1:100 steam AR for 25 min. with
citrate buffer, hrp rab labeled polymer for 30 min., DAB or AEC (I do this
on skin samples) for 10 min.  I have not tried the others.




From: ihcrg <@t> googlegroups.com [mailto:ihcrg <@t> googlegroups.com] On Behalf Of
Vaughan, Mary
Sent: Monday, April 16, 2007 6:58 AM
To: Histonet
Subject: [IHCRG] PDGF alpha, beta and receptors


good morning everyone-


I have a proposed project that involves staining of PDGF alpha, beta, and
the receptor for each. Does anyone have these protocols worked out? I'd
appreciate some help, thanks.





Best Regards,

Mary Vaughan HT (ASCP)

Research Specialist

Roswell Park Cancer Institute


Elm & Carlton Streets

Buffalo, N Y 14263

phone: 716.845.3006

FAX: 716.845.3489

email: mary.vaughan <@t> roswellpark.org


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