Dilution panel Re: [Histonet] CD34

[Gayle Callis]

I recommend doing a dilution panel, starting at 10 to 20 ug/ml on a 
positive control tissue.  This way you determine the correct antibody titer 
to fit your laboratory conditions.  The company product data sheet  should 
also give a recommended working concentration for their antibody, but a 
dilution panel is still a good idea.

A dilution panel is something we do as a standard procedure for all primary 
antibodies in order to optimize the working concentration.  This is 
important when one tries another source of the antibody as vendors may have 
a different clone than the one you have used before.


At 08:43 AM 4/18/2007, you wrote:
>What dilutions does anyone use for CD34? (for endothelial or
>
>  pan-endothelial markers?)   thx..........a wempren
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610