Dilution panel Re: [Histonet] CD34

Gayle Callis gcallis <@t> montana.edu
Wed Apr 18 09:54:52 CDT 2007

I recommend doing a dilution panel, starting at 10 to 20 ug/ml on a 
positive control tissue.  This way you determine the correct antibody titer 
to fit your laboratory conditions.  The company product data sheet  should 
also give a recommended working concentration for their antibody, but a 
dilution panel is still a good idea.

A dilution panel is something we do as a standard procedure for all primary 
antibodies in order to optimize the working concentration.  This is 
important when one tries another source of the antibody as vendors may have 
a different clone than the one you have used before.

At 08:43 AM 4/18/2007, you wrote:
>What dilutions does anyone use for CD34? (for endothelial or
>  pan-endothelial markers?)   thx..........a wempren
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610

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