[Histonet] alcian blue_ precipitation

[Tony Henwood]

Dissolve the dye in water first and then add diluted acid in water.
Eg for 1% alcian blue in 2.5% acetic acid:
1g dye in 50ml distilled water.
2.5ml acetic acid in 47.5ml water.
When the dye has dissolved, mix the two solutions

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of raghul
Sent: Tuesday, 17 April 2007 11:22 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] alcian blue_ precipitation


dear histonetters
   
  When i try to dissolve the alcian blue (sigma) powder in acidified
(ph2.5/ph1.0) dd water the stain isnt getting dissolved. When i try 1:1
alchol:distilled water adjusted to required pH its dissolving but isnt
staining any of mucin cells in positive control. please help
   
  raghul
  Medbiotech res. limited
  chennai, India

       
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