[Histonet] Alcian Blue Stabalizer

[Akemi Allison-Tacha]

--- Akemi Allison-Tacha <akemiat3377 <@t> yahoo.com> wrote:

> Date: Tue, 17 Apr 2007 12:22:07 -0700 (PDT)
> From: Akemi Allison-Tacha <akemiat3377 <@t> yahoo.com>
> Subject: RE: [Histonet] Alcian Blue 8GX and S from
> someone who made it to sell
> To: Sarah Jones <Sarah.Jones <@t> dako.com>
> 
> Sarah, I am speaking about incorporating a post
> stabalizer it the lq.solution of Alcian Blue not the
> synthetic powder dye.
> 
> Akemi
> 
> --- Sarah Jones <Sarah.Jones <@t> dako.com> wrote:
> 
> > John A. Kiernan was my basic source for the
> research
> > I did recently on
> > Alcian Blue.  In his book, Histological &
> > Histochemical Methods, he
> > states (referring to Alcian Blue 8GS, 8GX or 8GN),
> > "All these dyes are
> > supplied mixed with boric acid, sodium sulphate,
> and
> > dextrin." Also, in
> > an e-mail to the Histonet, Kiernan writes:  "A low
> > dye content such as
> > the 10-20% in your old ICI material is not
> > necessarily a bad thing for
> > Alcian Blue, and batches with high dye content
> > (70%+) often perform
> > badly.  It seems likely that the additives
> (dextrin,
> > boric acid, etc.)
> > are needed in adequate amount."  In addition,
> > quoting from Churukian et
> > al, 1972: "Minimum dye content for Commission
> > certification is 50%.  A
> > high dye content is at the expense of less
> > stabilizer.  Consequently,
> > purer samples may have reduced shelf-lives."  
> > 
> > Sarah Jones
> > 
> > 
> > 
> > -----Original Message-----
> > From: Akemi Allison-Tacha
> > [mailto:akemiat3377 <@t> yahoo.com]
> > Sent: Tuesday, April 17, 2007 11:49 AM
> > To: Sarah Jones
> > Subject: RE: [Histonet] Alcian Blue 8GX and S from
> > someone who made it
> > to sell
> > 
> > Very Interesting.  Chuck Churukian does not
> mention
> > any stabilizers in
> > his Special Stains book when making it up.  He was
> > in charge of
> > evaluating all dyes for the Biological Stains
> > Commission before
> > semi-retiring.
> >  We spoke on several collaborations for Special
> > Stains and he was one of
> > the people who the CAT hematoxylin
> > was named after.   
> > 
> > The image that is on the www.biocare.net site for
> > Alcian Blue pH 2.5 was
> > taken w/o the stabilizer.  I only started putting
> in
> > a stabilizer after
> > we started to sell it.
> > 
> > Akemi Allison-Tacha BS, HT (ASCP) HTL
> > President
> > Phoenix Lab Consulting & Staffing
> > Specializing in Histology, SS, IHC, & Microarray
> > Madison, WI
> > Tele:           (925) 788-0900
> > E-Mail: akemiat3377 <@t> yahoo.com
> > 
> > 
> > --- Sarah Jones <Sarah.Jones <@t> dako.com> wrote:
> > 
> > > Unfortunately, with Alcian Blue a higher dye
> > content doesn't
> > > necessarily mean a better dye.  Alcian Blue
> > absolutely NEEDS the
> > > stabilizers in order to work properly.  The
> higher
> > the dye content,
> > > the fewer stabilizers are in the dye.  This is
> an
> > exception to the
> > > usual rule about dyes and high dye content.
> > > Personally, I am finding that the synthetic
> Alcian
> > Blue dye renders a
> > > different blue than the Robin's egg blue we were
> > used to seeing over
> > > the years.  This blue seems to disappear if you
> > use a Hematoxylin
> > > counterstain, such as in the Alcian
> > Blue/PAS-Hematoxylin.
> > >
> > > Sarah Jones
> > > Dako North America
> > >
> > > -----Original Message-----
> > > From: histonet-bounces <@t> lists.utsouthwestern.edu
> > >
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]
> > > On Behalf Of Akemi
> > > Allison-Tacha
> > > Sent: Tuesday, April 17, 2007 11:19 AM
> > > To: histonet <@t> lists.utsouthwestern.edu
> > > Subject: [Histonet] Alcian Blue 8GX and S from
> > someone who made it to
> > > sell
> > >
> > > FYI to those of you who are not in the loop. 
> > Alcian Blue has not been
> > > procured in it's natural form for several years.
> > > There were too many
> > > fatalities during it's procurement from South
> > America.  This came to
> > > my attention in the late 90's.
> > >
> > > Anatech started creating it, in a synthetic form
> > some years ago. It
> > > was my understanding and I could be wrong, that
> > for awhile, Anatech
> > > was the only company who produced Alcian Blue to
> > pass the Biological
> > > Stains Commission evaluation process. It is my
> > understanding, there is
> > > only a 8GS being made now.  Again, I could be
> > wrong.
> > >
> > > In the past few years, Dudley Chemical, Aldrich,
> > Sigma, EM Science and
> > > some Chinese companies were able to produce
> > synthetic Alcian Blue,
> > > although the dye content varies with each
> > companies submission. You
> > > should always get a seal from the Biological
> > Stains Commission on each
> > > bottle of Alcian Blue powder.  If the label is
> not
> > on it, it has not
> > > passed their evaluation process. This only means
> > that it met it's
> > > minimum standard.  The Commision will not
> divulge
> > actual test results.
> > >
> > > When I started my R&D on Alcian Blue for
> Biocare,
> > Anatech had the
> > > highest dye content from the few companies that
> > sold it.  It is only
> > > my opinion, but they still have the best on the
> > market.
> > >
> > > Akemi Allison-Tacha BS, HT (ASCP) HTL
> > > President
> > > Phoenix Lab Consulting & Staffing
> > > Specializing in Histology, SS, IHC, & Microarray
> > Madison, WI
> > > Tele:         (925) 788-0900
> > > E-Mail:       akemiat3377 <@t> yahoo.com
> > >
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> > >
> > 
> > 
> > 
> 
>