[Histonet] Alcian Blue 8GX and S from someone who made it to sell

bhewlett <@t> cogeco.ca bhewlett <@t> cogeco.ca
Tue Apr 17 13:50:28 CDT 2007

> Akemi,

Alcian blue was NEVER available in any natural form.
It is and always has been, a synthetic dye first produced by ICI in the early fifties. The process of production
required some dangerous intermediates, that was why production ceased. Production now involves less dangerous
intermediates and hence more expense(naturally).


> FYI to those of you who are not in the loop.  Alcian
> Blue has not been procured in it's natural form for
> several years.  There were too many fatalities during
> it's procurement from South America.  This came to my
> attention in the late 90's.
> Anatech started creating it, in a synthetic form some
> years ago. It was my understanding and I could be
> wrong, that for awhile, Anatech was the only company
> who produced Alcian Blue to pass the Biological Stains
> Commission evaluation process. It is my understanding,
> there is only a 8GS being made now.  Again, I could be
> wrong.
> In the past few years, Dudley Chemical, Aldrich,
> Sigma, EM Science and some Chinese companies were able
> to produce synthetic Alcian Blue, although the dye
> content varies with each companies submission. You
> should always get a seal from the Biological Stains
> Commission on each bottle of Alcian Blue powder.  If
> the label is not on it, it has not passed their
> evaluation process. This only means that it met it's
> minimum standard.  The Commision will not divulge
> actual test results.
> When I started my R&D on Alcian Blue for Biocare,
> Anatech had the highest dye content from the few
> companies that sold it.  It is only my opinion, but
> they still have the best on the market.
> Akemi Allison-Tacha BS, HT (ASCP) HTL
> President
> Phoenix Lab Consulting & Staffing
> Specializing in Histology, SS, IHC, & Microarray
> Madison, WI
> Tele:     (925) 788-0900
> E-Mail:    akemiat3377 <@t> yahoo.com
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