[Histonet] component substitute in Spurrs

Gayle Callis gcallis <@t> montana.edu
Mon Apr 16 09:44:43 CDT 2007

Dear Phil,

Thanks for the update for the new component in Spurrs.  Please PDF the 
information to me privately.

  At 01:18 PM 4/13/2007, you wrote:
>Spurr's as such has not been discontinued, but one of its components has. 
>Which is good, it's my favorite resin for both plants and animals.
>ERL 4206, VCD, is no longer available, and ERL 4221 has been substituted. 
>4221 is Cycloaliphatic Epoxide Resin.
>The suppliers were (may still be) claiming that 4221 is a straight 1:1 
>substitute for 4206, but it isn't. Making Spurr's with the new component 
>is covered in:
>E. Ann Ellis
>Solutions to the Problem of Substitution of ERL 4221 for Vinyl Cyclohexene 
>Dioxide in Spurr Low Viscosity Embedding Formulations
>Microscopy Today, July 2006 14(4):32 ff
>I can a PDF of the article to anyone who wants it.
>>I had heard that Spurrs was discontinued.  Hopefully there is a 
>>substitute and contacting Energy Beam Sciences may be one way to find 
>>out.  Can anyone enlighten us on this?
>>Gayle Callis
>>Research Histopathology Supervisor
>>Veterinary Molecular Biology
>>Montana State University - Bozeman
>>PO Box 173610
>>Bozeman MT 59717-3610

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