[Histonet] Re: Fixative Formula
Kemlo.Rogerson <@t> waht.swest.nhs.uk
Mon Apr 16 02:11:17 CDT 2007
Formol 37-40% HCHO 10 ml
Dist Water 10 ml
Absolute ethanol 80 ml
"Tellyesniczky recommended a similar fluid to the last, containing 50 ml
each of formalin and acetic acid and 1000 ml alcohol. This fluid is to
be distinguished from the author's acetic dichromate formula."
R.D. Lillie 'Histologic Technic and Practical Histochemistry' 4th
Edition. McGraw-Hill 1976, ISBN 0-07-037862-2
Tellyesnic Fekete Opie Lillie's
Zky's & Lavin AAF
37-40% formaldehyde 5ml 10ml 5ml 10ml
Glacial acetic acid 5ml 5ml 5ml 5ml
Concentration 70% 70% 80% 95-100%
Amount 100ml 100ml 90ml 85ml
Page 34 of Lillie's esteemed volume.
"Trying to keep the old fixatives alive"
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