[Histonet] Demonstration of H. Pylori

[Marshall Terry Dr, Consultant Histopathologist]

I am replying on behalf of my technologists to say that their
pathologist likes ipox, though usually does with an H & E.
He asks for it when there is an appearance which suggests H. pylori
should be there but can't see it, or when he sees organisms but they are
not typical shape. (H. pylori occasionally has a coccal form).

One of the girls did a project comparing 6 or 7 stains and the best was
ipox,as one would expect. The next best was Giema, in that it gave the
best staining against background light staining, most consistently.
Silver stains were pretty hopeless. Tol. Blue easily overstained, and
others were too laborious.

Terry

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dolores
Townsend
Sent: 13 April 2007 14:46
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Demonstration of H. Pylori

Hello, Histonetters
I am taking a census: what stain do you/your pathologists prefer to
demonstrate H. Pylori?
How many of you do IHC?
How many do special stains, and if so, which one?
I do a Steiner here, which is time consuming, even in the microwave, and
seems to give variable results in staining intensity, so I'd like your
opinion as to what stain you prefer.
Thank you,
Dolores Townsend
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