[Histonet] When staining IHC's, are labs putting controltissue on every slide?

Thu Apr 12 08:53:31 CDT 2007

Yes, but I'm sure that our IHC volumne is no where near what your is.  I have thought about cutting and keeping some tonsil since that is our most used control but it's also easy to come by so I haven't really felt the need.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org

-----Original Message-----
From: Sebree Linda A. [mailto:LSebree <@t> uwhealth.org]
Sent: Thursday, April 12, 2007 8:58 AM
To: Tom McNemar; Richard Cartun; histonet <@t> lists.utsouthwestern.edu;
James F. Happel
Subject: RE: [Histonet] When staining IHC's,are labs putting
controltissue on every slide?

Tom,

Don't you go through a lot of control tissue but cutting it new each
time you need it?  We cut our control slides ahead of time and those
that are temperature sensitive we store at -20 degrees C with no
problem.  For instance, we generally cut our tonsil control 200 at a
time. and store some at RT and some at -20.

Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tom
McNemar
Sent: Thursday, April 12, 2007 4:57 AM
To: Richard Cartun; histonet <@t> lists.utsouthwestern.edu; James F. Happel
Subject: RE: [Histonet] When staining IHC's,are labs putting
controltissue on every slide?

We put a positive control on every slide.  We use the slides with the
red box for most of them.  The only ihc controls that we cut ahead are
the H. Pylori.  everything else we cut as needed.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Richard
Cartun
Sent: Wednesday, April 11, 2007 6:12 PM
To: histonet <@t> lists.utsouthwestern.edu; James F. Happel
Subject: Re: [Histonet] When staining IHC's, are labs putting
controltissue on every slide?

No, our positive control tissues are placed on separate slides.  We will
cut 15-60 slides at a time depending on how often the control is used
and the target protein's stability.

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Happel, James F." <JHAPPEL <@t> PARTNERS.ORG> 04/10/07 4:29 PM >>>
Good Afternoon All,

When staining IHC's, is anyone/everyone putting control tissue on every
slide?
Both + and - ?

James

-----Original Message-----
From: Liz Beitman [mailto:LBeitman <@t> cellmarque.com] 
Sent: Friday, March 23, 2007 12:19 PM
To: Happel, James F.
Subject: RE: [Histonet] Question about historical photographs

Hi James,

I just want to mention that we do have some posters of all the paintings
that adorn our catalog that I can send to you.  If you take a look in
our catalog (volume 7), page 79, it will give you an idea of some of the
posters.  Some are rather interesting from different points of view of
featured artists (Picasso, Klimt, and Michelangelo).  They might fit in
with your history presentation.  If you find any of interest, I would
look into either getting you an image or the actually poster.  Please
let me know.

Thanks,

Liz Beitman
Cell Marque
1-800-665-7284 x 8981
lbeitman <@t> cellmarque.com 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Happel,
James F.
Sent: Friday, March 23, 2007 7:49 AM
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] Question about historical photographs

Good Day,

I am putting together a presentation on the past, present and future of
histology/pathology and am looking for historical pictures, drawings,
advertisements, etc. to include in the presentation.  Does anyone have
images I
could use or know where I can find some?  Thank you.

James Happel
Massachusetts General Hospital

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