[Histonet] quantification of apical labeling in intestinal brushboarder

[Jonathan Wilson]

Hello Histonetters,
    I am looking for advice on quantify apical (brush boarder) 
immunolableing of intestine. The apical labeling is not uniform and I would 
therefore like a means of randomly sampling. I was wondering if it was 
acceptable to use an overlay grid and measure fluorescence intensity at the 
points where the grid lines intersected on randomly orientated micrographs. 
I know that this is used for stereology for morphometrics but haven't come 
across its use for this type of quantification (in my limited reading).
    My second problem that arises from this is what type of grid would be 
most appropriate and where I could get a copy or better yet how to produce 
one. Unfortunately, I do not have access to a good library (and therefore 
stereology books) and access to internet journals is limited.
Any help would be greatly appreciated.
Sincerely,
Jon


Jonathan Wilson (PhD)
Ecofisiologia CIMAR
Rua dos Bragas 289
4050-123 Porto Portugal
office 351 22 340 1809
lab 351 22 340 1834
fax 351 22 339 0608