[Histonet] When staining IHC's, are labs putting control tissue on every slide?

[Dawson, Glen]

I put a positive control on every slide with one negative run per case with the harshest (or most likely to cause staining retrieval/detection used on the case).  We go through obnoxious amounts of controls but I've found we have almost no repeats as it is hard for a pathologist to argue that an IHC did not work when the patient tissue is on the same slide as the positive control.  Also, just because the control for, say CD20, worked in position one, doesn't mean that the CD20 in position 32 didn't experience a machine malfunction or that it got the right reagents/antibody (especially when the workload calls for multiple vials of each).  Having the control on the same slide as the patient tissue also impresses CAP inpectors as it is, essentially, an on board validation for a working antibody.

Glen Dawson  BS, HT, & QIHC (ASCP)
IHC Manager
Milwaukee, WI

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Katri
Tuomala
Sent: Tuesday, April 10, 2007 9:23 PM
To: Happel, James F.; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] When staining IHC's,are labs putting control
tissue on every slide?


We service over 20 pathologists. Some of them want the control tissue on 
every slide (only one negative test and control is run per case with the 
harshest retrieval), some are happy with one control tissue for each 
antibody per run. We go through an awful lot of controls...

Katri

Katri Tuomala
Hamilton, Ontario, Canada

----- Original Message ----- 
From: "Happel, James F." <JHAPPEL <@t> PARTNERS.ORG>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, April 10, 2007 4:29 PM
Subject: [Histonet] When staining IHC's,are labs putting control tissue on 
every slide?


Good Afternoon All,

When staining IHC's, is anyone/everyone putting control tissue on every 
slide?
Both + and - ?

James

-----Original Message-----
From: Liz Beitman [mailto:LBeitman <@t> cellmarque.com]
Sent: Friday, March 23, 2007 12:19 PM
To: Happel, James F.
Subject: RE: [Histonet] Question about historical photographs

Hi James,

I just want to mention that we do have some posters of all the paintings
that adorn our catalog that I can send to you.  If you take a look in
our catalog (volume 7), page 79, it will give you an idea of some of the
posters.  Some are rather interesting from different points of view of
featured artists (Picasso, Klimt, and Michelangelo).  They might fit in
with your history presentation.  If you find any of interest, I would
look into either getting you an image or the actually poster.  Please
let me know.

Thanks,

Liz Beitman
Cell Marque
1-800-665-7284 x 8981
lbeitman <@t> cellmarque.com


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Happel,
James F.
Sent: Friday, March 23, 2007 7:49 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Question about historical photographs

Good Day,

I am putting together a presentation on the past, present and future of
histology/pathology and am looking for historical pictures, drawings,
advertisements, etc. to include in the presentation.  Does anyone have
images I
could use or know where I can find some?  Thank you.

James Happel
Massachusetts General Hospital





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