[Histonet] McMannas PAS Light Green

[Akemi Allison-Tacha]

Just a heads-up on Light Green Dye....  There has been
a problem with Light Green Dye for the past several
years. It ain't what it used to be!!  Atleast, that
was some inside info I was given from some reputable
sources from the Biological Stains Commission. 

As a solution, I developed a Fast-Light Green
Counterstain for Biocare's Masterpiece Line of Special
Stains.  My formulation is proprietary, but you can
purchase it from Biocare.  I used it for the PAS/Lt
Green for Fungus Stain and it looked beautiful.  Don't
worry--I don't getting any $$ from them since leaving.

Akemi Allison-Tacha BS, HT (ASCP) HTL
President
Phoenix Lab Consulting & Staffing
Specializing in Histology, SS, IHC, & Microarray
Madison, WI
Tele: 	(925) 788-0900
E-Mail:	akemiat3377 <@t> yahoo.com


 
--- Joe Nocito <jnocito <@t> satx.rr.com> wrote:

> Matt,
> we perform a PAS/Fungus on all toenails we gross
> (hence Joe the Toe). The 
> doctors I work for like out light green strong. I
> can't count how many times 
> they kicked back trays of slides that weren't
> stained dark enough. We have a 
> TissueTek stain line that we use for our special
> stains. We have an extra 
> container filled with 95% ETOH we use to rinse off
> the Fast Green. Every 
> time we did a quick rinse in water, the green always
> washed out. Personally, 
> the magenta color with the dark green just curls my
> toes up. I'm thinking of 
> painting my living room with those colors.
> 
> JTT
> 
> ----- Original Message ----- 
> From: <mjlco <@t> aol.com>
> To: <histonet <@t> lists.utsouthwestern.edu>
> Sent: Monday, April 09, 2007 8:50 AM
> Subject: [Histonet] McMannas PAS Light Green
> 
> 
> > Morning Histonetters,
> >
> > I am in need of everyones help. I am doing a
> McMannas PAS with light green 
> > on a fungus but am un-sure how much the light
> green it to show. Every time 
> > I have done this stain so far the light green has
> been extremly faint.
> >
> > I have been using a fungus in lung control.
> >
> > I have also been unable to find a photo of what
> this stain should look 
> > like.
> >
> > Thanks for all of your Help.
> > Matt Lunetta
> > Longmont United Hospital
> >
>
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