[Histonet] Bone saw
gcallis <@t> montana.edu
Tue Apr 10 13:49:47 CDT 2007
That Cabelas commercial butcher saw would probably do the job for you for
really large bones but remember that the actual passing of a saw blade
through a frozen bone will thaw the bone at the sawing site, simply due to
the heat created by the friction of the blade passing through the hard
bone. Is there no way the bone can be sawn BEFORE freezing, unless you do
not care about some bone loss. We always cut our samples before doing any
freezing. And the smaller the bone you need for undecalcified bone
sections means there will probably be more thawing and then refreezing on
the bone surface.
The MarMed saw would probably do the job too, but the Buehler Isomet is low
speed but requires a water cooling bath during the sawing process. The
Isomet is really best for small bones and not large bones as the cutoff
blades are not very large diameter, meaning much shallower cuts overall.
I hae seen hobby band saws used for cutting bone samples even when embedded
in PMMA and these saws sit on a countertop.
At 10:08 AM 4/10/2007, you wrote:
>I'm looking for a band saw or reasonable facsimile to cut large frozen
>blocks of tissue for subsequent frozen section microtomy - any ideas?
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