[Histonet] Plastic sections

[Sherwood, Margaret]

Liz,

I've never worked with JB-4, but soaking epon/epoxy blocks doesn't work as with
paraffin.  What you need to play with is the hardner component of the mix.

I know that sometimes other epoxys are needed for certain procedures, but my
experience is with Epon 812, which I prefer.  I am an electron microscopist, so
I work exclusively with plastic sections.

Peggy

Peggy Sherwood 
Lab Associate, Photopathology 
Wellman Center for Photomedicine (W224) 
Massachusetts General Hospital 
55 Fruit Street 
Boston, MA 02114-2696 
617-724-4839 (voice mail) 
617-726-6983 (lab) 
617-726-1206 (fax) 
msherwood <@t> partners.org 



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Wurdak,
Elizabeth
Sent: Monday, April 09, 2007 12:28 PM
To: Histonet
Subject: [Histonet] Plastic sections


Hello Histonetters,
We are having trouble with the JB-4 embedding medium.  No matter how
carefully we follow instructors provided by the vendor, EMS, our blocks turn
out to be too hard to section with sharp glass knives.  Should we cut back
on the catalyst, soak the blocks or switch to a different embedding medium
altogether?
Any help you can give me will be much appreciated.
Liz

Elizabeth Wurdak, PhD
Biology Department
Saint John¹s University
Collegeville, MN 56321
Tel: (320) 363-3177


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