[Histonet] Re: Slow Myosin heavy chain Abs
carl.hobbs <@t> kcl.ac.uk
Thu Apr 5 13:54:24 CDT 2007
Be careful with mouse embryonic tissues: they do not neccessarily express
the classical adult profile of immunostaining, imho.
The Novacastra Ab is great.
I just happen to use Chemicon's mab1628 which is equally good for pwax
mouse sections, optimally after HIER.
I assume that you will be using adult mouse muscle to confirm the fidelity
of your embryonic positivity?
If yes, you must, again imho, also use an anti fast MHC Ab: Sigma's MY32 is
very good for me.
Demonstrate that anti MHC fast/slow Abs work as expected on a
particular adult mouse muscle ( one that has a mixed population of fast/slow
fibres) then use anti fast AND slow MHC on your embyronic sections on the
same muscle, as your control.
Also, imho, you must use an anti Desmin Ab, for the record.
Just my opinion........
Please come back with your thoughts..and then results!
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