[Histonet] double-staining with two rabbit antibodies, Zenon-kit

[Yves Heremans]

Dear All,

We need to double-stain with two antibodies made in rabbit and would prefer to use fluorescent detection for both. I know that Molecular Probes has Zenon kits for differential fluorescent labeling of rabbit antibodies, allowing you to use multiple antibodies from the same species, however these kits require that you know the concentration of the antibodies to be labeled. Since our rabbit antibodies are not affinity-purified but consist of whole serum, is there a way around this and use the Zenon kit anyway ? 

Yves