[Histonet] Re: effects of elevation on immunohistochemistry
Rene J Buesa
rjbuesa <@t> yahoo.com
Thu Sep 28 09:44:59 CDT 2006
This is a Physics issue! At higher elevation atmosferic pressure is lower and the water boils at less than 100ºC so any HIER done in an "open" container, like heating in the MW oven or using a steamer or a water bath, will be affected because 100ºC cannot be reached.
HIER should be done in a pressurized environment (like a pressure cooker). There was a paper published in the Jorunal of Histotechnology not too long ago (I do not have the exact reference) dealing with Her-2 Neu tests at high elevation.
Any step requiring a certain temperature in an open container, will be affected. Other steps temperature independednt will not be affected.
Hope this will help you!
Lisa Manning <manningl <@t> inspection.gc.ca> wrote:
Does anyone have any experience or knowledge about the effects of
elevation on immunohistochemistry?
Thanks kindly, Lisa Manning
Lisa Manning R.T, B.Sc.
Canadian Food Inspection Agency
National Centre for Foreign Animal Disease
1015 Arlington Avenue, Suite T2300
Winnipeg, Manitoba R3E 3M4
PH. (204) 789-2087
email: manningl <@t> inspection.gc.ca
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