[Histonet] Tissue in Paraffin

Tony Henwood AnthonyH <@t> chw.edu.au
Tue Sep 26 17:59:20 CDT 2006

Since the tissue is already processed to wax, you can safely remove the
cassettes from the wax and let them harden to room temp. Then to embed,
place them in molten wax for 30 min or so to remelt the tissue ready for
embedding. The tissue is already infiltrated with wax so no harm can
come to them.


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Heckford, Karen - SMMC-SF
Sent: Tuesday, 26 September 2006 10:21 PM
To: Histonet (E-mail)
Subject: [Histonet] Tissue in Paraffin

Good Morning my fellow Histonetters,  I was wondering how long you would
leave  tissue unembedded after pulling them out of paraffin.  My
processor is broke down.  I would greatly appreciate the feedback.
Karen Heckford HT (ASCP) CE
Lead Histology Technician
Histology/Pathololgy Department
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
Fax: 415-750-8123
email: kheckfor <@t> chw.edu

This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.

More information about the Histonet mailing list