AW: [Histonet] Sentinel node protocol

[Gudrun Lang]

 If the sentinel comes in for a frozen section, half of it is done for the
frozen and half of it is saved for the paraffin-process. We cut three
fozen-levels. With breast-sentinels we do one HE -first section- and one HE
- last section-, between we cut in 250 µm steps. These sections are stained
with anti-Pancytokeratin. Melanoma-sentinels are cut in the same way and
stained with Pan-Melanoma-Cocktail.
Recently we heard about a quick-frozen-stain for Pancytokeratin with the
Envision-detection kit, and want to try it soon. If someone can give me
input on it, i will be glad.

Gudrun Lang



-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Mildred
Fail
Gesendet: Dienstag, 26. September 2006 15:32
An: Histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Sentinel node protocol

Good morning,
       At present we are doing IHC for AE1/AE3, CK7, and CAM5.2. I would
like to know the IHC sentinel protocols performed in other labs. Thanks 

Rena Fail


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