[Histonet] Tracheal whole mount staining

[sohail ejaz]

Dear All
   
  
  I am currently screening DKK1 transgenic mice for their effect on tracheal vasculature using the whole mount immunoflouresence.
   
  I am using Cd31 antibody for that and everything is going well when i do it with retinal whole mount staining but when it comes to trachea, i cant see any blood vessels. 
   
  Is there anyone who could kindly guide me a complete protocol for whole mount immunoflouresence of trachea.
   
  Thanks
   
  Dr.Sohail Ejaz


histonet-request <@t> lists.utsouthwestern.edu wrote:  Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu

You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

1. re: Honey as a fixative (Carl Hobbs)
2. RE: flame Dako (sheila adey)
3. RE: flame Dako (Ron Martin)


----------------------------------------------------------------------

Message: 1
Date: Sat, 23 Sep 2006 18:55:04 +0100
From: "Carl Hobbs" 
Subject: [Histonet] re: Honey as a fixative
To: "Histonet" 
Message-ID: 
Content-Type: text/plain; charset="iso-8859-1"

No
..it is a preservative, not a fixative. Tim Morken does in fact mention
this en passant. However, just thought I’d underline the fact.
Carl




------------------------------

Message: 2
Date: Sat, 23 Sep 2006 16:01:31 -0400
From: "sheila adey" 
Subject: RE: [Histonet] flame Dako
To: mauger <@t> email.chop.edu, Histonet <@t> pathology.swmed.edu,
DOOLEEO <@t> shands.ufl.edu
Cc: MAGILJ <@t> pathology.ufl.edu
Message-ID: 
Content-Type: text/plain; format=flowed

I agree totally. We are switching to Biogenex. (i6000)
Our job is tough enough with out worrying about having the products we need. 
Just curious, were you using the Artisan? Thay's what we have.

Sheila Adey


>From: "Joanne Mauger" 
>To: ,
>CC: MAGILJ <@t> pathology.ufl.edu
>Subject: [Histonet] flame Dako
>Date: Fri, 22 Sep 2006 10:31:39 -0400
>
>Lets all flame DAKO! What's the problem? They are discontinuing so many
>products, and we have been waiting for months to receive our orders!! I
>was trying to be patient, but I have just about had it!! I have switched
>to many LabVision products. Get with it, DAKO, or you will lose
>customers!
>
>That feels better,
>
>Jo Mauger
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_________________________________________________________________
Don’t waste time standing in line—try shopping online. Visit Sympatico / MSN 
Shopping today! http://shopping.sympatico.msn.ca




------------------------------

Message: 3
Date: Sat, 23 Sep 2006 17:46:08 -0400
From: "Ron Martin" 

Subject: RE: [Histonet] flame Dako
To: "'sheila adey'" ,
, ,

Cc: MAGILJ <@t> pathology.ufl.edu
Message-ID: <000001c6df59$adfa8e40$2ea2ac45 <@t> D6WRV2B1>
Content-Type: text/plain; charset="us-ascii"

I miss my old Biogenex i6000!!!!!!!

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of sheila adey
Sent: Saturday, September 23, 2006 4:02 PM
To: mauger <@t> email.chop.edu; Histonet <@t> pathology.swmed.edu;
DOOLEEO <@t> shands.ufl.edu
Cc: MAGILJ <@t> pathology.ufl.edu
Subject: RE: [Histonet] flame Dako

I agree totally. We are switching to Biogenex. (i6000)
Our job is tough enough with out worrying about having the products we need.

Just curious, were you using the Artisan? Thay's what we have.

Sheila Adey


>From: "Joanne Mauger" 
>To: ,
>CC: MAGILJ <@t> pathology.ufl.edu
>Subject: [Histonet] flame Dako
>Date: Fri, 22 Sep 2006 10:31:39 -0400
>
>Lets all flame DAKO! What's the problem? They are discontinuing so many
>products, and we have been waiting for months to receive our orders!! I
>was trying to be patient, but I have just about had it!! I have switched
>to many LabVision products. Get with it, DAKO, or you will lose
>customers!
>
>That feels better,
>
>Jo Mauger
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_________________________________________________________________
Don't waste time standing in line-try shopping online. Visit Sympatico / MSN

Shopping today! http://shopping.sympatico.msn.ca


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 34, Issue 29
****************************************


 			
---------------------------------
Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business.