[Histonet] Job Opportunity

Thu Sep 21 19:54:45 CDT 2006

We have a full time histology position available at Roche Palo Alto in California.  The position requires working with a variety of laboratory animal tissues for pre-clincal drug safety studies, performing wet tissue trimming to staining for routine paraffin processing, special stains, frozen sections, IHC and other specialized techniques.  Interested person please send resume to shirley.jen <@t> roche.com.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Thursday, September 21, 2006 10:10 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 34, Issue 25

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Today's Topics:

   1. Fwd: Re: [Histonet] Histonet down??? (Rene J Buesa)
   2. Fwd: Re: [Histonet] honey as a formalin substitute (Rene J Buesa)
   3. Fwd: Re: [Histonet] Histonet down??? (Rene J Buesa)
   4. RE: honey as a formalin substitute (Rittman, Barry R)
   5. Re: Histonet Digest, Vol 34, Issue 24 (Steven2146 <@t> aol.com)
   6. Re: Tunel staining with acid decalcified samples (Johnson, Teri)
   7. RE: Technovit 9100 Kit (Jacqueline D. Garfield)

----------------------------------------------------------------------

Message: 1
Date: Thu, 21 Sep 2006 09:35:28 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Fwd: Re: [Histonet] Histonet down???
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20060921163529.68713.qmail <@t> web61220.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Fellow Histonetters:
  I though you may want to read this e-mail I received from Linda Margraf, to know the reason behind what kept all of us without our "daily dosis of the addictive Histonet".
  Regards
  René J.

LINDA MARGRAF <LINDA.MARGRAF <@t> childrens.com> wrote:
  Date: Thu, 21 Sep 2006 08:47:42 -0500
From: "LINDA MARGRAF" <LINDA.MARGRAF <@t> childrens.com>
To: <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Histonet down???

It turns out the problem is with the university email system. They changes some filters and have been blocking all outgoing email from the server. I was still receiving messages from Histonet so it took a while to realize what the problem was. They are working on it and I hope it will be fixed soon. You are on the list and will start getting mail when the filters are fixed. Thanks for your patience. Linda M Histonet administrator

>>> Rene J Buesa 09/19/06 9:24 AM >>>
Histonet server:
Myself and several user have not received any e-mails via Histonet for several days. Also the archival pages have not been updated since 9/13/06. Is Histonet out of service? Please advise René J. Buesa

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Message: 2
Date: Thu, 21 Sep 2006 09:46:47 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Fwd: Re: [Histonet] honey as a formalin substitute
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20060921164647.42194.qmail <@t> web61217.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:  Date: Thu, 21 Sep 2006 09:29:55 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] honey as a formalin substitute
To: "Olszewski, Dawn" <Dawn.Olszewski <@t> SGMC.ORG>, 
histonet <@t> lists.utsouthwestern.edu

  Dawn:
  The article was published in the Journal of Histotechnology, September 2006 issue (vol.29, No.3, pages 173-9).

  I read the article and was not really impressed by it because:
  1-the honey used came from a source that would not be readily available to everybody, which means that tests with other types/sources of honey will be necessary;
  2-there are no comparative methods, no statistical analysis of the results or documented comparisons with standard procedures, and
  3- if to substantiate the results we have to judge by the photomicrographs, they are of a very poor quality and what reflect is poor processing. That poor processing could be due to the processing protocol itself or to the fixation the authors claim to have perfomed with honey.
  If that is the case, the procedure does not seem to be very promising. I would have require the authors to rewrite some aspects of the paper before publication.
  Just my opinion based in what I read.
  René J.

"Olszewski, Dawn" <Dawn.Olszewski <@t> SGMC.ORG> wrote:
  Help!!!
We have a student in our lab who has been asked to write a summary of the article "The effectiveness of honey as a substitute for formalin in the histological fixation of tissue". Has anyone read this article ( or know where this article can be found) or know anything about this subject matter? If so, any and all info would be appreciated. Thanks in advance. 

Dawn Olszewski
SGMC 
Valdosta, Ga
dawn.olszewski <@t> sgmc.org

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Message: 3
Date: Thu, 21 Sep 2006 09:47:23 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Fwd: Re: [Histonet] Histonet down???
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20060921164724.33591.qmail <@t> web61212.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:  Date: Thu, 21 Sep 2006 09:35:28 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Fwd: Re: [Histonet] Histonet down???
To: histonet <@t> lists.utsouthwestern.edu

  Fellow Histonetters:
  I though you may want to read this e-mail I received from Linda Margraf, to know the reason behind what kept all of us without our "daily dosis of the addictive Histonet".
  Regards
  René J.

LINDA MARGRAF <LINDA.MARGRAF <@t> childrens.com> wrote:
  Date: Thu, 21 Sep 2006 08:47:42 -0500
From: "LINDA MARGRAF" <LINDA.MARGRAF <@t> childrens.com>
To: <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Histonet down???

It turns out the problem is with the university email system. They changes some filters and have been blocking all outgoing email from the server. I was still receiving messages from Histonet so it took a while to realize what the problem was. They are working on it and I hope it will be fixed soon. You are on the list and will start getting mail when the filters are fixed. Thanks for your patience. Linda M Histonet administrator

>>> Rene J Buesa 09/19/06 9:24 AM >>>
Histonet server:
Myself and several user have not received any e-mails via Histonet for several days. Also the archival pages have not been updated since 9/13/06. Is Histonet out of service? Please advise René J. Buesa

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Message: 4
Date: Thu, 21 Sep 2006 11:48:00 -0500
From: "Rittman, Barry R" <Barry.R.Rittman <@t> uth.tmc.edu>
Subject: RE: [Histonet] honey as a formalin substitute
To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>,	"Olszewski, Dawn"
	<Dawn.Olszewski <@t> SGMC.ORG>,	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<EA1FDD2A141B7448B4B1AFFFCAC08DE40716F079 <@t> UTHEVS1.mail.uthouston.edu>
Content-Type: text/plain;	charset="iso-8859-1"

Rene
I was in process of also commenting on this paper to the Journal but perhaps here is as important.

Rene - I agree with all the points that you have written.
To me the images of their formalin fixed tissue do not represent any acceptable level of fixation anywhere I have worked. 
The major problem that I have is that this journal is peer reviewed is it not? My question then is who reviewed this? Based on the article I personally would not have accepted this in its current form for publication - based on the images alone, let alone the other points. 
I am not familiar with the work of the authors but even if they are new to the field I believe that when any papers are submitted for publication they should receive a critical review. This is in everyone's interest. It encourages authors to look very critically at their submitted work and lets them know how their peers will look at the articles. People should be given every encouragement to submit papers but should also be prepared for a very critical review. We all have tunnel vision when we write papers and benefit from others reviewing these. Just my opinion also. Barry

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, September 21, 2006 11:30 AM
To: Olszewski, Dawn; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] honey as a formalin substitute

Dawn:
  The article was published in the Journal of Histotechnology, September 2006 issue (vol.29, No.3, pages 173-9).

  I read the article and was not really impressed by it because:
  1-the honey used came from a source that would not be readily available to everybody, which means that tests with other types/sources of honey will be necessary;
  2-there are no comparative methods, no statistical analysis of the results or documented comparisons with standard procedures, and
  3- if to substantiate the results we have to judge by the photomicrographs, they are of a very poor quality and what reflect is poor processing. That poor processing could be due to the processing protocol itself or to the fixation the authors claim to have perfomed with honey.
  If that is the case, the procedure does not seem to be very promising. I would have require the authors to rewrite some aspects of the paper before publication.
  Just my opinion based in what I read.
  René J.

"Olszewski, Dawn" <Dawn.Olszewski <@t> SGMC.ORG> wrote:
  Help!!!
We have a student in our lab who has been asked to write a summary of the article "The effectiveness of honey as a substitute for formalin in the histological fixation of tissue". Has anyone read this article ( or know where this article can be found) or know anything about this subject matter? If so, any and all info would be appreciated. Thanks in advance. 

Dawn Olszewski
SGMC 
Valdosta, Ga
dawn.olszewski <@t> sgmc.org

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

---------------------------------
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_______________________________________________
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------------------------------

Message: 5
Date: Thu, 21 Sep 2006 12:49:05 EDT
From: Steven2146 <@t> aol.com
Subject: [Histonet] Re: Histonet Digest, Vol 34, Issue 24
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <c83.1fa5aa2.32441c81 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"

Hi...
I believe I saw a reference to that in the latest issue of NSH  magazine.

Steven Lee

------------------------------

Message: 6
Date: Thu, 21 Sep 2006 11:52:38 -0500
From: "Johnson, Teri" <TJJ <@t> Stowers-Institute.org>
Subject: [Histonet] Re: Tunel staining with acid decalcified samples
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<C28BAF593DC3314E9C0F3A50191C2E78044B814A <@t> EXCHKC03.stowers-institute.org>

Content-Type: text/plain;	charset="us-ascii"

(formerly "Yeh glad to be back & ??")

Amy, I'm not surprised you are having difficulty with TUNEL staining on acid decalcified samples. You are denaturing the DNA with your decalcifier.

You might look into using EDTA instead, and with mouse femurs it shouldn't take more than a week for adequate decalcification.

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64110

------------------------------

Message: 7
Date: Thu, 21 Sep 2006 12:54:13 -0400
From: "Jacqueline D. Garfield" <JGarfield <@t> lifecell.com>
Subject: RE: [Histonet] Technovit 9100 Kit
To: "Lin Bustamante" <LBUSTAMANTE <@t> cvm.tamu.edu>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<FC9D561C53102849A6F3CDD0C063A72F01944E11 <@t> exch1.lifecell.biz>
Content-Type: text/plain;	charset="us-ascii"

Lin,

You can find this product at Electron Microscopy Sciences (EMS).  Their address and phone number:

	321 Morris Road
	Box 251
	Fort Washington, PA 19034

	(215) 646-1566
	(800) 523-5874

	www.emsdiasum.com

Regards,
Jackie Garfield

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lin Bustamante
Sent: Thursday, September 21, 2006 11:16 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Technovit 9100 Kit

Please help me to find the distributor of this kit in the USA. I tried many companies with no luck. Thank you. Lin.

Lin S. Bustamante, B.Sc.; HT(ASCP)
Histology Lab
Dept. of Veterinary Anatomy and Public Health
Texas A&M University
College Station, TX 77843-4458

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End of Histonet Digest, Vol 34, Issue 25
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