[Histonet] Re: Tunel staining with acid decalcified samples

[Johnson, Teri]

(formerly "Yeh glad to be back & ??")

Amy, I'm not surprised you are having difficulty with TUNEL staining on
acid decalcified samples. You are denaturing the DNA with your
decalcifier.

You might look into using EDTA instead, and with mouse femurs it
shouldn't take more than a week for adequate decalcification.

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64110