[Histonet] Re: Histonet Plasmacytosis

[chiggerson <@t> memhosp.com]

Joyce,

We use CD138 pre-dilute from Cell Marque on the Ventana Benchmark XT. They 
have several different sizes of pre-dilute and concentrate. Our 
pathologists love it. We use hidradenitis suppurativa as a positive 
control (it has a lot more plasma cells than tonsil and is easier to get 
than plasmacytoma). 

Procedure:

Mild cell conditioning (Tris/EDTA buffer, pH 8) for 30 minutes.
AB incubation 32 min @ 37 degrees.
with Amplification

Hope this helps.

Cindy 

Message: 1
Date: Tue, 19 Sep 2006 13:07:38 -0400
From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
Subject: [Histonet] Immunoperoxidase for plasmacytosis
To: "Histonet \(E-mail\)" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
 <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEEAE7 <@t> sjhaexc02.sjha.org>
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We have a pathologist who is insisting on a stain for plasma cells. Thus 
the previous question about MGP kits. 
 
Second question, do any of you do CD138 for plasma cells. If so, would you 
please share your source for the antibody and your procedure?
 
Thanks,
Joyce
 
Joyce Weems
Pathology Manager
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
404-851-7376 - Phone
404-851-7831 - Fax