[Histonet] True Blue chromogen

Perry, Margaret Margaret.Perry <@t> sdstate.edu
Tue Sep 12 11:27:54 CDT 2006

I am in need of some clarification on information in Chris van der Loos
book "Immunoenzyme Multiple Staining Methods".  On page 45 in the chart
he lists TMB HRP substrate as producing a green color.  In KPL's catalog
it lists TMB as blue and I talked to their tech service and they also
said it turns blue.  Do you have to do something to the TMB to turn it
green or does it turn green automatically with IHC ?  Are there any
other chromogens that turn green?  


Thanks in advance for your help.


Margaret Perry HT(ASCP)

South Dakota State University

Vet Science

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