[Histonet] tissues in cleaning cycle

[MARY T HODGES]

   a tissue left in the cleaning cycle is completetly taben back to water
   and needs to be reprocessed for formalin. just place the tissue on ice
   water  ans  see  how  fast the tissue is white. it a test for fixation
   that  ols  techs  used  long  ago to test processing if it is white in
   20-30 secs there is no fixation.

   I would just reprocess the block and have before .it comes out great

   tere hodges
       ______________________________________________________________

     From:  "Anne Van Binsbergen" <vanann702 <@t> skmc.gov.ae>
     To:  "Rene    J    Buesa"    <rjbuesa <@t> yahoo.com>,   "Till,   Renee"
     <TillRenee <@t> uams.edu>,<histonet <@t> lists.utsouthwestern.edu>
     Subject:  RE: [Histonet] tissues in cleaning cycle
     Date:  Wed, 6 Sep 2006 17:28:45 +0400
     >I  totally  disagree-  my  vip5's  cleaning cycle does xylene then
     absolute  and  then has a water wash cycle. If I left a block in my
     wash  cycle it would end up in water so I would certainly reprocess
     it  from  at  least the 95% station!! If I put it straight into the
     xylene I would put water into my xylene!!!
     >Just my 5cents worth here in the desert
     >Annie
     >
     >-----Original Message-----
     >From:                    histonet-bounces <@t> lists.utsouthwestern.edu
     [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]   On  Behalf  Of
     Rene J Buesa
     >Sent: 06 September 2006 17:01
     >To: Till, Renee; histonet <@t> lists.utsouthwestern.edu
     >Subject: Re: [Histonet] tissues in cleaning cycle
     >
     >Renée:
     >    A  tissue  left  in  the cleaning cycle is one that is already
     dehydrated  and  in  the  "antemedium"  (xylene if that is what you
     use).  That is "normal procedure" if you want to "reinfiltrate" the
     tissue,  so  your  next  step  would  be to put it BRIEFLY in clean
     xylene and transfer it to be infiltrated with paraffin.
     >    Very  likely  tat  your  tissue  can be put in the last xylene
     station and through the paraffin stations.
     >   René J.
     >
     >"Till, Renee" <TillRenee <@t> uams.edu> wrote:
     >    Hello.  Any  suggestions  on what to do with tissues that were
     accidentally
     >left  in  the  processor  during the cleaning cycle? I would think
     they need
     >to be re-infiltrated with paraffin at the very least. I don't know
     if
     >our processor will let us do anything but a full processing run.
     >
     >
     >
     >Renee' Till, HT
     >
     >Research Assistant
     >
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     >Fax (501)364-3161
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