[Histonet] re:anti mouse CD31

[PALMER Jason (SVHM)]

We have been using BD Biosciences rat anti mouse CD31 (Clone MEC13.3 / #553370) for a while now on FFPE mouse tissue  (dermis and connective / adipose tissue mainly). On the whole, it works pretty well for us, though there is the odd tissue where labelling is very weak or absent and we can't quite seem to work out why. Nothing special about our protocol - proteinase K 8 mins, block, primary at 1:150, rabbit anti rat Ig - biotin, vector ABC elite, DAB....

Jason

Jason Palmer
Bernard O'Brien Institute of Microsurgery
42 Fitzroy St, Fitzroy Victoria 3065
Australia
tel +61 3 9288 4018
fax +61 3 9416 0926
email: jason.palmer <@t> svhm.org.au




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Message: 18
Date: Thu, 7 Sep 2006 10:46:15 -0500
From: "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>
Subject: [Histonet] Anti mouse CD31
To: histonet <@t> pathology.swmed.edu,
	histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
	<OF6F8E410C.1491C7D2-ON862571E2.00568297-862571E2.0056AB3D <@t> abbott.com>
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If anyone has any new ideas on CD31 that works in murine tissue, I'll give
you a million dollars. (If you'll take a check . . . . . post dated)

Wacky Jackie

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