[Histonet] Anti mouse CD31

Bugelski, Peter [CNTUS] PBugelsk <@t> CNTUS.JNJ.COM
Thu Sep 7 12:36:45 CDT 2006

I've had good luck with murine anti-CD31 in paraffin sections of human
xenografts in tissue fixed with a modification of Beckstead's zinc.  

Tris 12.1 g
Calcium chloride 0.5 g
Zinc acetate  10 g
formalin 13.5 mL
Distilled H2O to 900 mL

adjust pH until clear

Bring to 1000 mL

We published using this this fixative in Tang Y et al.  CAncer Res 65:3193,

Peter J. Bugelski, Ph.D., FRCPath
Senior Research Fellow and 
Head of Experimental Pathology
Centocor R-4-2
200 Great Valley Parkway
Malvern, PA 19355

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