AW: [Histonet] bone marrow procedure

[Gudrun Lang]

 Hi Michele,
I think it is an important point to know, how thick and long the cores are.
We process bone marrow trephines with 3-4 mm diameter and about 30 mm
length. Therefore we let them fix in 8% NBF until the next day of receiving,
next day morning we decal them in a bought mixture with formic acid and
formaldehyd for 8 hours. Then they are washed in running water for 15 min
and put in the usual processing cycle in the VIP. Together with the Ventana
IHC system we have HE and IHC on the second day after receiving.
Smaller samples will probably require shorter times.

Gudrun
Akh Linz, Austria


-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Margiotta,
Michele
Gesendet: Donnerstag, 07. September 2006 17:49
An: histonet <@t> pathology.swmed.edu
Betreff: [Histonet] bone marrow procedure

Hi All,
Our pathologist is wondering how most labs are processing bone marrow bxs.
Are you doing a rapid or slow decal and for how long?  If someone could
share their procedure, that would be most helpful.

Thanks,
Michele



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