[Histonet] (no subject)

[Belmadani, Souad]

Hi,
im trying to stain endothelium in mice hearts, i used the anti CD31 from abcm and it did not work , now im using the FVW from DAKo again its not working. im using the antibody at 1;200 dilution overnight and no staining at all 
its formalin paraffine section and im using the citrate buffer ph 6 as antigen retrieval ....
any ideas why its not working 
i apprecite your help
thanks 
souad