[Histonet] (no subject)
sbelma <@t> lsuhsc.edu
Fri Sep 1 16:13:09 CDT 2006
im trying to stain endothelium in mice hearts, i used the anti CD31 from abcm and it did not work , now im using the FVW from DAKo again its not working. im using the antibody at 1;200 dilution overnight and no staining at all
its formalin paraffine section and im using the citrate buffer ph 6 as antigen retrieval ....
any ideas why its not working
i apprecite your help
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