[Histonet] B5 Immuno
BMolinari <@t> heart.thi.tmc.edu
Fri Sep 1 09:34:15 CDT 2006
An investigator asked me about this and having very little experience
with immunos I come to you.
She found an article where myocardial sections were fixed in B5 without
formalin (is there a B5 without formalin?) and then stained with several
polyclonal and monoclonal antibodies. She wanted to know why B5?
Wouldn't 10% NBF be just as effective? I did some archiving and read
that some have had success with zinc formalin and B Plus. Also I do know
of the hazards of B5 so we will not be using it in my lab, but just
Betsy Molinari HT (ASCP)
Texas Heart Institute
6770 Bertner Ave.
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