[Histonet] TRAP staining on undecalcified bone

[Tiffany Pitts]

Hello all,
I am trying to do TRAP staining on undecalcified mouse bones cut from 
Methylmethacrylate embedded blocks. My problem is that I cannot seem to find 
a protocol that works well and/or is on the market any more. Does anyone 
know of a protocol I can use or (even better) a kit? Our in-house protocol 
is light on details and I'm not sure if I'm doing it right.
Thank you

Tiffany Pitts
Department of Urology
University of Washington
Seattle, WA