[Histonet] Timms stain in fresh frozen cryosections

[Anna Elisse Beaudin]

Hello,

   I would like to do Timm's stain in fresh frozen cryosections of mouse
brain.  The only protocol I could find involves incubating the sections
O/N in a dessication chamber with highly toxic H2S gas (created by
adjusting a 0.1% Na2S solution to pH 7.3) prior to rehydration followed
by classic timms staining protocol.  I was wondering if anybody had any
other protocols and/or the best way to do this without poisoning myself
and my lab mates :)  I really appreciate any help!

Thanks in advance,
Anna Beaudin
Division of Nutritional Sciences
Cornell University