[Histonet] Steamed About Steamer

[Marshall Terry Dr, Consultant Histopathologist]

By one of those odd coincidences, the next e-mail I received after this one was from my wife who is house sitting whilst her son and family are away, because they have the builders in.
It included inter alia:

"The rain  stopped at midday and it is a lovely afternoon.  The roofers are steaming nicely!"

Shall I ask her to find out the temperature of boiling water on the roof, and how long they took to get to "steaming"?

Have a good weekend everyone.

PS Wonder if there are any rice cooker firms listening - I feel a pit>hole coming on.
 

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire
 England
        terry.marshall <@t> rothgen.nhs.uk

-----Original Message-----
From: Fred Underwood [mailto:funderwood <@t> mcohio.org]
Sent: 27 October 2006 13:22
To: amosbrooks <@t> gmail.com; histonet <@t> lists.utsouthwestern.edu; Marshall
Terry Dr, Consultant Histopathologist
Subject: RE: [Histonet] Steamed About Steamer


I'm happy to see that this has become a "Nan" issue.

>>> "Marshall Terry Dr,Consultant Histopathologist"
<Terry.Marshall <@t> rothgen.nhs.uk> 10/27/2006 5:40 AM >>>
I am intrigued by this discussion.
I have been using rice steamers for 42 years.
I use them to cook rice, oddly enough:-)
They have been used with great frequency - I love my Indian food.
Never ever, have I had one that decreased in performance.
The only problem has been that the inner pan gets gradually pitted by
the salt until it holes.

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire
 England
        terry.marshall <@t> rothgen.nhs.uk 

-----Original Message-----
From: Amos Brooks [mailto:amosbrooks <@t> gmail.com] 
Sent: 27 October 2006 01:06
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] Steamed About Steamer


Hi,
     I have only used 2 brands of steamer so far but there's no reason
another can't work as well. Certainly steamers can wear out as one user
has
pointed out. I have seen this, the heating element stops getting the
temperature high enough. As this is a new steamer that shouldn't be
the
problem.
     It's possible that the steaming rack is too far from the element
leaving the steam enough time to cooldown before it reaches the slides.
That
is pretty much a design flaw if that is the case. If so there's nothing
else
for it but to replace it. Try to get something compact that allows
enough
space for whatever container you are using to hold the slide rack.
     It is possible that the vents are allowing the steam out too
quickly.
That could prevent the steam from heating the buffer up enough. If so
you
could cover some of the holes (Try duct tape at first, you can figure
out
something more permanent if it works.
     To make sure the buffer gets up to the right temperature, I have
holes
drilled in the top of my steamers just big enough to fit a thermometer.
This
way I know the buffer is hot enough to put the slides in and when the
buffer
gets back up to the right temperature after the slides are added. This
is
great because you can directly monitor the temperature of the solution
in
real time thruought the retrieval process. (Try that in a microwave or
pressure cooker)
     In response to the gentleman that was having trouble with
E-Cadherin,
we have found an extra 10 min. produces a stronger antibody reaction.
This
works for ER & PR too. I am sure we'll find more if we actually get
time to
test more antibodies.
Good Luck
Amos Brooks
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