[Histonet] decalcification question

[Rittman, Barry R]

Jeanine
In my experience, solutions that combine a fixative and decalcification
agent are very slow. 
In general the problem with a prolonged decalcification e.g. because the
specimen is large, is the risk of maceration due to some reversal of the
bonds formed during fixation. If you are using EDTA you can periodically
remove the specimen from the solution and replace in the fixative
overnight, i.e. a "refixation".
This is much more rapid than using the combination solution and the
results, in my hands have been as good.

What end result do you wish to achieve?
-immunohistochemistry, paraffin or frozen sections, gross examination. 
Have the heads been treated for example by perfusion to show blood
vessels?
Thanks
Barry



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Bartlett, Jeanine (CDC/CCID/NCZVED)
Sent: Friday, October 20, 2006 8:05 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] decalcification question
Sensitivity: Confidential

Hi all,

We are need of information regarding the best decal procedure for whole
ferret heads.  We have been using a commercial decal/fixative
combination but it is a very slow process.  Any suggestions will be
greatly appreciated.

Thanks,

Jeanine Bartlett, BS, HT(ASCP)
Centers for Disease Control and Prevention
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590 
jeanine.bartlett <@t> cdc.hhs.gov

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