[Histonet] cytospin slide staining

[Bruijntjes, J.P. (Joost)]

Hi

I've done anti cleaved caspase on ethanol (70%) fixed tissues and I used
an EDTA-buffer pH 8.0 with good results. The first time I used this
antibody I've tried the citrate buffer pH 6.0 with no results at all,
but changing to pH 8.0 gave me better results.

Joost Bruijntjes
TNO Quality of Life
Tel: +31 30 6944480
Zeist
Holland
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Dahui You
Sent: Wednesday, October 18, 2006 8:08 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cytospin slide staining


Hello, histonetters:
Has anyone done cytospin slide staining before? What antigen retrieval
method you used?
I am staining bronchoalveolar lavage fluid slides with anti-active
caspase3 and none of my experimental and control slides showed positive.
I am wondering if that is real or I did it wrong since I don't have any
positive control slides for this antibody and I never did this kind of
staining before.
Any suggestion will be greatly appreciated!
Have a nice day.


Dahui You
LSU


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