[Histonet] cytospin slide staining

[Dahui You]

Hello, histonetters:
Has anyone done cytospin slide staining before? What antigen retrieval method you used?
I am staining bronchoalveolar lavage fluid slides with anti-active caspase3 and none of my experimental and control slides showed positive.
I am wondering if that is real or I did it wrong since I don't have any positive control slides for this antibody and I never did this kind of staining before.
Any suggestion will be greatly appreciated!
Have a nice day.


Dahui You
LSU