[Histonet] trichrome staining

[Malcolm McCallum]

According to Humason's Animal Tissue Techniques (5th ed.), Pg 126.  You can use Masson Trichrome Staining method with any general fixative.  Pollak Rapid Method (pg 128) can also use any general fixative, although mercuric chloride will improve formalin fixed tissues.  Finally, you can use Gomori's one-step Trichrome as it is intended for use with neutral buffered formalin (pg 129).
 
Hope that helps!
 
VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org <http://www.herpconbio.org> 
A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation
and the World Congress of Herpetology.
 
Malcolm L. McCallum
Assistant Professor
Department of Biological Sciences
Texas A&M University Texarkana
2600 Robison Rd.
Texarkana, TX 75501
O: 1-903-223-3134
H: 1-903-791-3843
Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html
 

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of White, Lori
Sent: Tue 10/17/2006 2:03 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] trichrome staining



Does anybody have a method for trichrome staining that does not use
picric acid or mercury for fixation?  References would be wonderful if
you have them.

Thanks,

Lori White

Peterborough, Ontario









Important Notice - This electronic transmission is strictly confidential to Peterborough Regional Health Centre and intended to be delivered only to the addressee. Information contained within the transmission, is protected by legal, professional and/or other privilege. If you are not the intended addressee, or not authorized by the intended addressee to receive this transmission, you must not retain, disclose in any manner, copy, or take any action in reliance on this transmission. If you have received this transmission in error, as a courtesy, please notify the sender as soon as possible and then delete or destroy this message.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet