[Histonet] cryosections of rat bone

[Nancy W. Troiano]

We use the 4X slides from instrumedics and flash twice (flash once, leave 
the slide in the cryostat chamber while you cut another slide or two and 
then reflash the original slide - make sure you wait 30-40 seconds between 
flashes or you'll ruin your unit).  Also, remove the tape after the slide 
warms up slightly or place the slide on the back of your hand to warm it up 
before pulling off the tape.  This procedure should help but so far we've 
found that we can never get all of the bone to transfer and we've tried a 
lot of different combinations to get that bone to stick to the slide!