[Histonet] freezing kidney bx

[DeBrosse_Beatrice]

We used to snap freeze kidney bx's for direct IF in liquid nitrogen.

Beatrice DeBrosse-Serra
HT(ASCP)QIHC
Allergan, Inc.
2525 Dupont Drive RD-2A
Irvine, CA 92612
714-246-5116


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Thursday, October 12, 2006 3:48 PM
To: Annette Featherstone; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] freezing kidney bx

We freeze ours in the cryostat.

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Featherstone, Annette" <AFeatherstone <@t> KaleidaHealth.Org> 10/11/06
10:06 AM >>>
When freezing kidney biopsies for the purpose of direct
immunofluourescence what is the recommendation....snap freezing in
liquid nitrogen or freezing in a cryostat? 
Thanks

Annette Featherstone HT/MLT ASCP, MT (HEW)
Supervisor Anatomic Pathology
Kaleida Health, Buffalo General Hospital
100 High Street
Buffalo NY 14203
716-859-2625
FAX: 716-859-1853



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