[Histonet] cryosectioning rat bone

[John Baker]

Hi All,   Does anyone out there in histoland get good cryo-sections of 
rat femur or tibia?  I get great 5-7 micron sections of mouse bone 
using the CryoJane Tape System.  Whether longitudinal or cross the 
cortical bone will not laminate and comes off with the window.  I 
certainly don't get sections at all using the anti-roll plate.  I have 
a Hacker-Bright OTF cryostat and use t-c blades.  Any suggestions are 
greatly appreciated.  Thanks in advance.  John

John A. Baker
The University of Michigan
Orthopaedic Research Laboratories
Histology Unit
109 Zina Pitcher Place, 2218 BSRB
Ann Arbor, MI 48109-2200
734-936-1635