[Histonet] Monoclonal antibody to Beta-galactosidase

[Kim Merriam]

I have been using a rabbit polyclonal from Abcam on (ab616) @ 1:800, HIER overnight at 60-65C in Biocare Reveal or BORG (don't use the BOG on tissues that are really fatty, like skin or brains - they will fall off!).  The BORG gives better staining, but the Reveal is acceptable if using fatty tissues.
 
Kim Merriam
Cambridge, MA



----- Original Message ----
From: Jeanne Estabel <je2 <@t> sanger.ac.uk>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Wednesday, October 11, 2006 5:09:32 AM
Subject: [Histonet] Monoclonal antibody to Beta-galactosidase


Hi, 



I need to find a monoclonal antibody to Beta-galactosidase working on
FFPE mouse tissue.

Could you give me some advice?



Thanks



Jeanne Estabel, PhD

MGP Histologist, Team 109

Wellcome Trust/Sanger Institute

Hinxton

CB10 1SA

UK



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