[Histonet] Reduction of Ice Crystals in Large Specimens

[Grant Steyer]

I routinely perform sectioning of whole mice and other large specimens.  The
best protocol I have found is to perfuse the mouse with a mixture of sucrose
and DMSO before embedding in OCT and freezing in liquid nitrogen.  Is anyone
aware of other protocols that may be more effective in reducing ice crystals
in large specimens? Thanks in advance for your help - Grant