[Histonet] Re: Superfrost slides-Histonet question

[Stephen Peters M.D.]

Hi Ion,
   
  In our Leica !850's I use a blade angle of 5 with our low profile blades. I know I 
  would not have much luck at a negative angle.
   As far as curling tissue, in my experience the best temperature for cutting
   most tissus (except fat) is the warmest temp that the tissue will cut 
  without crumpling. Try starting  to cut a touch on the warm side. Press a piece 
  of cryostat temp metal ( I use my over-chuck blocks for this) on the block face a
   few seconds at a time until the tissue starts to cut. When the tissue is just 
  cold enough to cut it will float off in a flat sheet. This is why the sections in my 
  videos lie so flat. It is largely do to the correct temperature. As we cut colder and colder we get more and more curling and shattering. If you are starting with a very 
  cold block  warming with the thumb will get you down to the same temp. But if it
   takes more than a few seconds to get your section, warming will need to be 
  repeated as the temp will quickly fall back down to the core temp of the block.
   Thanks for visiting my web site.  Gail thanks for the complement it means a lot coming from you.
   
   
   
   


Stephen Peters M.D. 
Vice Chairman of Pathology
Hackensack University Medical Center 
201 996 4836
 
Pathology Innovations, LLC 
410 Old Mill Lane, 
Wyckoff, NJ 07481 
201 847 7600 
www.pathologyinnovations.com