[Histonet] blocking peptide
tiang yeepeng
yeepengtiang <@t> hotmail.com
Mon Oct 2 19:06:02 CDT 2006
Dear all,
I am currently using a cell-cycle marker, which is the Ki-67 made in
the mouse for immunostaining. However, I need to do a western blot to
validate my host protein in HeLa cells and I need a blocking peptide.
The company which I bought my Ab from doesnt sell the peptide and I
have to outsource from other companies. However, I found that the
peptides are sold with the Ki-67 Ab made in other hosts and no one is
actually selling blocking peptide for Ki-67 made in mouse. Will there
be any problem if I use the peptide which is meant for Ki-67Ab made in
other hosts (instead of mouse in which my Ab is made from)? Thanks in
advance!
With regards,
TIANG YEE PENG
Department of Medical Microbiology,
Faculty of Medicine,
University of Malaya.
Correspondence address:
Molecular Diagnostics Research Laboratory,
(c/o: Clinical Diagnostics Laboratory)
4th floor, East Tower,
University of Malaya Medical Centre,
50603 Pantai Valley,
Kuala Lumpur,
MALAYSIA.
Tel: (+6)03-79492205
Fax: (+6)03-79492818
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